Detection of minus strand RNA of HCV in liver tissues of patient with hepatitis C and hepatocellular carcinoma by RT-PCR RT-PCR检测肝炎和肝癌组织中的HCV复制中间体
Methods Using digoxin labelled plus strand and minus strand HCV RNA probes, an in situ hybridization technique suitable for detecting HCV RNA in the peripheral blood mononuclear cells ( PBMC) was established. 方法应用地高辛素标记HCVRNA正链及负链探针,建立原位杂交方法检测外周血单个核细胞(PMBC)中的HCVRNA。
In the cancer tissues of a same case, if minus strand RNA of HCV was positive, its signal intension had no significant difference from that of HCV plus strand RNA. 在同例肝(癌)组织中,若负链HCVRNA阳性,则其信号强度与正链基因无明显差别;
HCV RNA in PBMC was extracted and tested for both plus and minus strand by RT-PCR. All of these serum were anti-HCV and HCV RNA positive. 所选检测对象血清标本抗-HCV及HCVRNA均为阳性。
The minus strand of HCV RNA was located within cytoplasm. 原位杂交法证实细胞内存在负链RNA,也位于胞浆中。